·1634 -
维普资讯hp-www.cqvip.con
MODERN ONCOLOGY,Nov. 2007,VOI. 15, NO. 11
原发性肝细胞癌患者外周血单个核细胞中HBV-DNA含量检测的意义高应林，赵友云，王春香，乐惠荣
Clinical significanceof HBV DNA concentrationin peripheral blood mononuclearcells of patientswithprimaryhepatocellularcarcinoma
GAO Ying lin,ZHAO Youyun,WANG Chun xiang, LE Huirong
Department of Clinical Laboratories, Hubei Prowincial Hospital of Traditional Chinese Medicine, Wuhan 430061,China.
[Abstract] Objective: To investigate the integration of hepatitis B virus( HBV) DNA in peripheral blood mononu-clear cells(PBMCs) in the patients with hepatocellular carcinoma(HCC) , and to explore the mechanism of HBV -DNA in PBMCs in HCC patients. Methods: Fluorescent quantitative polymerase chain reaction(FQ PCR) was ap-plied to detect HBV - DNA both in serum and in PBMCs of 32 patients with HCC( HCC group) and 45 patients with chronic hepatitis B( CHB group) ,primers and probe were designed for HBV C gene. Results: HBV DNA positive rate in serum of HCC group and CHB group were 62. 5% (20/32) and 46. 7% (21/45) respectively, the average lev els of HBVDNA concentration were1o and 104copies/ml respectively;HBVDNA positiverates in PBMCs of HCC group and CHB group were 87. 5% (28/32) and 51. 1% (23/45) respectively ,the difference was sig nificant(P< 0. OI ), the average levels of HBV DNA concentration ( only HBV - DNA positive patients) were 10L3 and 10.5copies/ml respectively HBVDNA psitiverate in PBMCs of HCC group was higher than that in serum(87. 5% VS 62. 5% ,P <0. 05). HBV DNA of HCC patients and CHB patients were detected both in their serum and in PBMCs ,the HBV - DNA concentration in PBMCs were all lower than that in serum, the correlation of HBV DNA concentration were significantly positive(r =0. 9018, P <0. 01 in HCC group, and r = 0. 9025, P < O. O1 in CHB group,respectively). Conclusion:The integration of HBV DNA exists in PBMCs; by comparison with liver tissue,detecting HBV DNA concentration in PBMCs has the advantage with no harm as risky signal of predic ting HCC.
[ Key words] primary hepatocellular carcinoma :peripheral blood mononuclear cells; HBV - DNA
Modem Oncology 2007,15(11) :1634 ~ 1635
【摘要】
目的：了解原发性肝细胞癌(PHCC)患者外周血单个核细胞（PBMC)中乙型肝炎病毒核酸（HBV
DNA)的整合情况，以揭示其在HCC发病机制中的作用。
方法：应用荧光定量聚合酶链反应(FQ-PCR）技术
检测32例HCC患者和45例慢性乙型肝类（CHB）患者血清和PBMC中HBV-DNA含量，引物和探针设计选择HBVC区的DNA序列。结果：HCC组和CHB组患者血清中HBV-DNA阳性率分别为62.5%（(20/32）和
2和105.06±
copies/ml,PBMC 中 HBV -DNA 阳
46.7%（21/45）、HBV-DNA均值（不含阴性）分别为10
性率分别为87.5%（28/32)和51.1%（23/45）（P<0.01）、HBV-DNA均值（不含阴性）分别为10+51."和
104.0 ±1.00
copies/ml;HCC组血清和PBMC中HBV~DNA阳性率相比仁统计学差异(62.5%比87.5%，P<
0.05）;HCC组和CHB组血清和PBMC中HBV-DNA同时阳性时，J:HBV-DNA含量均呈正相关（P< 0.01）。结论：HCC患者PBMC中存在HBV-DNA整合的现象；作为临床预测HCC发生的风险指标，PBMC
中HBV-DNA含量的检测与穿刺肝组织相比具有轻创或无创的优点。【关键词】原发性肝细胞瘤；外周血单个核细胞；HBV-DNA
【文章编号】16724992（2007)11163402
【中图分类号】R735.7
【文献标识码】A
原发性肝细胞癌（PHCC）是全世界主要健康问题，在我国其死亡率已占恶性肿瘤死亡的第三位"]。研究显示HCC 发生与乙型肝炎病毒（HBV)的感染关系密切[2),HBV核酸（HBV-DNA)整合于宿主细胞是PHCC的发病机制之一[3]。
【收稿目期】【作者单位】【作者简介】
2007 03 06
湖北省中医医院检验科
潮北
武汉
430061
高应林（1958－），男，朗北广济人，主管技郏，主要从事临床基因诊断工作。
我们为了解HCC患者外周血单个核细胞(PBMC)中是否存在HBV-DNA的整合情况，以揭示其在HCC发病机制中的作用，选择了32例HCC患者和45例慢性乙型肝炎（CHB）患者作为研究对象，应用荧光定量合酶链反应（FQ-PCR）技术检测其血清和PBMC中HBV-DNA含量。
材料与方法 1
研究对象
1.1
32例HCC患者来自我院2005年1月至2006年12月肝炎病房，诊断来源于核磁共振、CT和手术后的病理检查，其