第35卷，第5期 2015年5月
光谱学与光谱分析 Spectroscopy and Spectral Analysis
Vol. 35 ,No. 5 -ppl177-1181
May,2015
The Application of Quality Identification in Honey by
PhotoacousticSpectroscopy
TAO Wen-ting,YUAN Ping*,GUO Wen-juan,LIU Jian-en
Key Laboratory of Atomic and Molecular Physics & Functional Materials of Gansu Province, College of
Physics and Eletronic Engineering, Northwest Normal University , Lanzhou 730070, China
Abstract The photoacoustic spectrum of glucose, sucrose and honey solutions in the visible range are meas+ ured by using the single-light photoacoustic spectrometer , and are compared with the spectra from spectropho-tometry method . The spectral characteristics of the above solutions show that the spectral background intensity and spectral profile have some differences for different kinds of solutions . The spectra of the three kinds of so-lutions all have strong peak value at 485 and 655 nm , but the intensity ratios between the two peaks are differ-ent. Besides , there are characteristic peak at 475, 576 and 630 nm for glucose , and the sucrose has apparent characteristic peak at 632 nm , these characteristic peaks can be used for detecting whether the natural honey has been added glucose or sucrose . By comparing two kinds of spectrum of the same solution , the intensity o photoacoustic spectrum is more responsive to the wavelength, indicating photoacoustic spectrometry has a higher sensitivity in the test of material composition .
KeywordsPhotoacoustic spectroscopy ; Glucose; Sucrose; Honey ; Characteristic peak
中图分类号：0433.4
Introduction
文献标识码：A
D0I; 10. 3964 /i issn. 1000-0593(2015 )05-1177-05
is the popular traditional natural tonic . Honey quality identifi-cation has become one of the important topics related food safety , Sivakesav and Irudayaraj5-9] identified the adulteration
Photoacoustic spectroscopy is an effective way for detec-ting material composition. The PAS only measures the ab-sorption of light to the material and is weakly influenced by the measuring wavelength and particle size distribution , so it is suitable for the measurement of solid , liquid and gas . There are many superiorities of photoacoustic spectroscopy + such as wide measurement range , no need for sample preprocessing + nondestructive measurement, low sample consumption and high detection sensitivityl--) , the research on the liquid com-position by this technique is attracting more attention .
Material composition and content are common concerned problem , particularly for food, such as milk , cooking oil, honey and drinks . Honey with high edible and medical value
Received : 2014-05-28 ; accepted : 2014-09-25
of honey with glucose , sucrose and IS by using fourier trans-form infrared spectrometer combining with attenuated total reflectance . Paradkar et al .lol using fourier transform raman spectroscopy studied adulterants such as cane and beet invert in honey . Cotte et al.Cul analyzed honey adulteration with syrup by nuclear magnetic resonance technology - According to the royal jelly has special physiological functions in active ingredient can judge whether royal jelly adulteration of honey by means of immunochemical detection[12) . At present, it is very few of the work on researching the honey composition by photoacoustic spectroscopy .
In this paper , the photoacoustic spectra of glucose solu-tion, sucrose solution and many kinds of honey solution are
Foundation item; National Natural Seience Foundation of China under Grant (11475139, 11365019)
Biography : TAO Wen-ting, (1986—), female, a graduate student at Collgeg of Physics and Electronic Engineering , Northwest Normal
University
e-mail : taowt21@ 163 .com
* Corresponding author
e-mail : yuanp@ nw nu -edu .cn