第18卷第4期 2018年8月
环境与能源
过程工程学报
The Chinese Journal of Process Engineering
Vol.18 No.4 Aug.2018
D0I: 10.12034/j.issn.1009-606X.217354
Microbial characterization of micropolluted sourcewatercontaining microcystin
pretreatmentwithbiologicalaeratedfilter Wenyi ZHANG'，YichaoWANG'，Qingqing CAI12
1. School of Environmental & Safety Engineering, Changzhou University, Changzhou, Jiangsu 213164, China 2. Jiangsu Longhuan Environmental Science Co., Ltd., Changzhou, Jiangsu 213127, China
Abstract: The microbial characteristics of biodegradation of organic compounds containing nitrogen and microcystin LR (MC-LR) was researched with the biofilm of biological aerated filter (BAF) reactor surface as the research object, by the means of microscopic examination, phospholipid fatty acid (PLFA), high-throughput sequencing and others. The results showed that the microbial community on the biofilm of BAF was extremely rich in the micro-polluted source water of low carbon source,formed the microbial community consists of aerobic bacteria, sulfate reducing bacteria and other bacteria. In the first 2 weeks, the microflora on the biofilm had 17 major genera and 6 species. In the 3 to 4 weeks, the microflora will increase to 43 major genera and 14 species. There also were protists such as nematodes,paramecium and daphnia.The dominant species were Sphaerotilus (2.41%~24.58%),Aeromonas(4.16%~12.59%),Cloacibacterium
(1.85%~12.39%)，Aquabacterium(1.53%~6.76%)，Hydrogenophaga
(1.12%~5.9%), Methyloversatilis (0.53%~1.52%), Rhodobacter (0.09%~1.39%), etc. In addition, the content of bacillus increased from the initial 0.16% to 1.97% after the addition of T1 degrading bacteria, which showed that
bacilus grew rapidly and became dominant population after adding T1 degrading bacteria, thus enhancing the degradation of MC-LR.
Key words: drinking water sources; biological aerated filter; microorganism
BAF工艺预处理含微囊藻毒素微污染水源的微生物特征
王逸超，蔡庆庆12
张文艺!·，
1.常州大学环境与安全工程学院，江苏常州213164： 2.江苏龙环环境科技有限公司，江苏常州213127
摘要：以曝气生物滤池载体表面的生物膜为研究对象，通过镜检、磷脂脂肪酸(PLFA)测定、高通量454测序等手段研究了生物膜降解含氨有机物和微囊藻毒素-亮氨酸(MC-LR)的微生物特征。结果表明，低碳源的微污染水源中水生物膜的微生物群落极为丰富，形成了由好氧细菌组成的微生物群落，运行前2周有6大门类17大种属，3~4周增加到14大门类43大种属，还有线虫、草履虫、水蛋等原生动物：优势菌属有球衣菌属Sphaerotilhus(2.41%~24.58%)、气单胞属Aeromonas(4.16%~12.59%)、黄杆菌属Cloacibacterium(1.85%~12.39%)、水杆菌属Aquabacterium(1.53%~6.76%)、噬氢菌属Hydrogenophaga(1.12%~5.9%)、嗜甲基菌属Methyloversatilis(0.53%~1.52%)、红杆菌属Rhodobacter(0.09%~1.39%)等.此外，投加0.16%T1菌剂芽孢杆菌后其含量增至1.97%，表明投加T1降解菌后芽孢杆菌迅速生长并成为优势种群，可强化降解MC-LR
关键词：水源水；瞩气生物滤池；微生物
中图分类号：X524：X703
文献标识码：A
文章编号：1009-606X(2018)04086606
886886
基金项目：国家自然科学基金资助项目(编号：41571471)：国家"十三五"科技重大专项基金资助项目(编号：2017ZX07202002) 作者简介：张文艺(1968-)，男，安省安庆市人，博士，教授，研究方向：水污染控制与生态修复，E-mail:zwy@czu.edu.cn
引用格式：张文艺，王逸超，蒙庆庆。BAF工艺预处理含徽囊藻毒素微污染水源的微生物特征，过程工程学报，2018，18(4):866-871.
aining microcystin pretreaatment with
Zheng W Y, Wang Y C, Cai Q Q,Microbial characterization of micro polluted se
biological aerated filter (in Chinese), Chin. J. Process Eng., 2018, 18(4): 866871, DOI: 10.12034/jissn.1009-606X.217354.
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