第30卷第3期 2010年9月
化学传感器 CHEMICALSENSORS
基于纳米粒子吸附的甲基对硫磷传感器研究
刘激娟12*，谭正初1，钟兴附12
(1.湖南省农科院茶叶研究所，潮南长沙410125）(2.湖南省茶叶检测中心，湖南长沙410125）
Val. 30, No. 3 Sept.2010
搞要：将ZrO,纳米粒子用于有机磷传感器的构造，制成了电沉积ZrO,粒子层/金电极(ZrOyAu)电流型传感器，可直接测定茶汤中有机磷农残的含量。以甲基对硫磷为分析目标物，研究了传感器的主要响应特性、选择性及再生性能，考察了溶液pH值、电化学参数等因素对传感器分析性能的影响。结果表明，该有机磷传感器在10~500ng/mL浓度范围内对目标分析物有线性响应，检出限为2.0ng/mL。该传感器灵敏度高，非特异性吸附小，再生性好，使用简单，所用的二氧化错缩米粒子层制备简单、操作方便，具有较大的应用潜力。关键词：二氧化错纳米粒子；生物传感器；有机磷；甲基对硫磷
Studiesonanovelparathion-methylbiosensorbasedon
nanoparticleabsorption
Liu Shu-juan'', Tan Zheng-chu', Zhong Xing-gang"
(1. Tea Research Institute, Hunan Academy of Agricultural Science, Changsha 410125, China)
(2. Hunan Tea Test Center, Changsha 410125, China)
Abstract: In this paper, a novel biosensor for detecting organophosphorus has been prepared. Firstly, & zirconia film is electrodeposited onto the cleaned surface of gold electrode. Then organophosphonus can be immobilized on these zirconia nanoparticles by adsorption. Putting the electrode into O.1 mol/L KCl solution, we can quantitatively deter-mine the organophosphorus concentration according to the electrochemical response intensity of the modified elec-trode, Taking parathionmethyl as example, the effects of solution pH value and electrochemical parameters on the analytical performance have been investigated, and the response character, reproducibility aad interferences of the biosensor are also tested. The proposed biosensor in optimal conditions has a linear range of 10 ~500 ng/mL for organophosphorus detection, and its detection limit is 2.0 ng/ml, Moreover, the proposed biosensor has advantages in high sensitivity, low nonspecific absorption and advantageous regeneration process. The process for preparation of zirconia nanoparticles is simple and convenient.
Key words: zirconia; biosensor, organophosphorus; parathionmethyl
0引言
由于人们在防治病虫害过程中不合理和超剂量使用化学农药，导致了粮食、蔬菜、茶叶等农产品中的农药残留间题，不仅影响了农产品的品质，也危害人类的健康。传统上有机磷农药分析方法主要是利用气相色谱、薄层色谱、高效液相
本文系湖南省农科院科技创新项目资助*通讯联系人，E-mail:juannet66@126.com
万方数据
色谱以及各种光谱联用技术(11。虽然这些方法的选择性、灵敏度和准确度高，但需要昂贵的仪器设备，样品的前处理过程繁项、费时，并且对分析人员的技术水平要求很高，不适于现场检测。而且仪器分析法多为检测干物质中的农残含量，不能准确反映人体实际摄入的农残含量。
与传统分析技术相比，有机磷传感器快速检