第36卷第3期 2016年9月
化学传感器 CHEMICALSENSORS
基于纳米空心Pt-Au复合物共修饰癌胚抗原
免疫传感器的研究
朱宇萍，刘丹丹
（内江师范学院化学化工学院，四川内江641112）
Vel. 36, No. 3 Sept.2016
摘要：利用领性模板法制备纳米空心Pt-Au复合物。将恭酚/硫通过键合作用修饰在玻碳电极表面，并利用硫堇的氨基将表面吸附有亚酒酸根负离子的空心纳米Pt-Au复合物修饰在茶酚/硫董膜表面，以此固载癌旺胚抗体，从面构建新型癌胚抗原免疫传感器。以循环伏安法对电极构建过程及性能进行测试，结果表明，此免疫传感器对癌胚抗原有良好的电流响应。在优化实验条件下，癌胚抗原(CEA)的浓度在0.1~80ng/mL内
有良好的线性关系，检测限为0.03ng/ml。该免疫传感器有望应用于临床上CEA的检测。关键词：癌胚抗原：免疫传感器；空心纳米材料；硫董；Pt-Au
Study onthe carcinoembryonic antigenimmunosensor modified by
Pt-Auhollownanocomposites
Zhu Yu-ping'. Liu Dan-dan
(College of Chemistry and Chemical Engineering, Neijiang Nomal University, Neijiang 641112, China) Abstract: The PtAu hollow nanocomposites was prepared by template sacrifice. By bonding action, the nafion/ thionine was modified on the surface of the glassy carbon electrode. The PtAu hollow nanocomposites on which surface the selenite anion was adsorbed, were modified on the surface of the nafion/thionine film by the amidogen of the thionine, to immobilize antibody CEA to construct the CEA immunosensor, The conconstruction process and performance of the electrode were measured. The result showed that the immunosensor had well current response for CEA. Under the optimal conditions, in the concentration of CEA of 0.1~80 ng/mL,the linear relation was well, and
the detection limit was 0.03 ng/mL. The immunosensor could be used for the clinical deteetion of CEA. Key words: CEA; immune sensor, hollow nanocomposites; thionine; PtAu
价格昂责、RIA自身的放射污染及使用条件的限
0引言
癌胚抗原(CEA)是一种具有黏附性、可溶性的异性糖蛋白分子。研究发现肿瘤患者血清中 CEA浓度较之正常人有所上升，临床上CEA可作为肺癌、乳腺癌、肠癌等多种癌症的肿癌标志物之，通过患者血清中CEA浓度可检测肿癌变[1]。
当前已有酶联免疫吸附法(ELISA)、时段辨别荧光免疫法(TRFIA)、放射性免疫法(RIA)、化学发光免疫法(CLIA)21等检测CEA。但CLIA检测仪器
制。电化学免疫传感器拥有制作简单、高灵敏度、检测快速便捷、稳定性好等优点，电化学免疫传感器对CEA的定量测定有着更高的利用前景。
纳米材料是近年来应用较多的新型材料。纳米材料拥有比表面效应、量子尺寸效应、小尺寸效应和宏观量子隧道效应[3-4]，许多文献报道用纳米材料构建免疫传感器。以纳米复合物作为基底，固载生物分子能够加大固载量、提高反应活性；另外纳米材料生物相容性可保持吸附的抗体生物活性、无毒性和生物可降解性(5]。将
基金项目：四川省教育厅科研项目（15ZA0292）
*通信联系人，Tel;13696067037，Email：judy20060830@163.com 万方数据