植物营养与肥料学报2010,16(6)：1472-1478 Plant Nutrition and Fertilizer Science
连年翻压绿肥对植烟土壤微生物量及
酶活性的影响
刘国顺"，李正"，敬海霞，叶协锋"，史宏志，王永"，2，杨超3，常栋（1河南农业大学，国家烟草栽培生理生化研究基地，烟草行业烟草栽培重点实验室，河南郑州450002；
2郑州经济技术开发区，河南郑州450016；3重庆市烟草公司，重庆400023）
摘要：通过3年田间定位试验，研究连年翻压绿肥对植烟土填微生物量碳、氮及酵活性的影响。结果表明，连年髓压绿肥能提高土壤微生物量碳、氮及土填脉酶、酸性磷酸酶、点糖酶、过氢化氢酶的活性，且随翻压年限的增加面增加。整个生育期，翻压3年绿肥的处理与对照相比微生物量碳、氮分别提高31.0%～67.1%、23.0%～145.1%；土壤脉酶、酸性磷酸酶、蔗糖酵、过氧化氢酶活性分别提高34.4%~51.9%、11.0%~18.6%、58.0%~172.7%、 24.0%～50.0%，表明翻压绿肥后土壤生物过程活跃，利于有机物质的转化和烤烟正常生长所需的营养供应。动态变化特征表明，翻/压绿肥1、2、3年的各处理微生物碳、氮均在团棵期出现峰值，土壤脉酶、酸性磷酸酶、过氧化 09 填脲酶、酸性磷酸酶、过氢化氢酶活性分别提高51.9%、14.2%、30.6%。此时正值生育旺期，利于烟株生长发育，说明连年翻压绿肥后培肥土壤效果显著。土壤微生物量C、N和酶活性能灵敏反映土壤肥力的变化，可作为评价土壤质量的生物学指标。
关键调：绿肥；土壤微生物量碳；土壤微生物量氮；土衰酶活性
中图分类号：S142；S154
文献标识码：A
文章编号：1008-505X(2010)06-1472-07
Effects of consecutiveturnover of greenmanures on soil microbial biomass
andenzymeactivity
LIU Guo-shun', LI Zheng", Jing Hai-xia', YE Xie-feng', SHI Hong-zhi', WANG Yong', YANG Chao', CHANG Dong
(I Henan Agricultural University/National Tobacco Cultiration and Physiology and Biochemistry Research
Centre/Key Laboratory for Tobacco Cultivation of Tobaco Industry, Zhengzhou 450002, China 2 Economic and Technological Development Zone of Zhengzhou, Zhengzhou, 450016, China;
3 Tobacco Company of Chongqing，Chongqing 400023,China）
Abstract: A consecutive 3-year field experiment was conducted to study the effects of incorporation green manure on soil microbial biomass carbon, microbial biomass nitrogen and soil enzyme activities. The results showed that in-corporation green manure could increase soil microbial biomass C, N and the activities of soil urease, acid phospha-tase( ACP), sucrase, and catalase with the increasing of the returning age. Compared with the control, the con-tents of soil microbial biomass C and N were increased by 31. 0%67. 1% and 23. 0%-145. 1% , and the activities of soil urease, ACP, invertase and catalase by 34. 4%51. 9% , 11. 0%18. 6 % , 58. 0%172. 7% , and 24. 0%-50. 0% after three year' consecutive turnover of green manure, respectively, suggesting that the tumover of or ganic matter was facilitated and more nutrients were released to meet the demanding of crop growth. The dynamical changes of soil microbial biomass C,N and the enzyme activity within tobacco development period showed that the maximum of microbial biomass C and N was observed at the rosett stage, while the peak of soil enzyme activities was appeared at the busy stage of flue-cured tobacco. Compared with the control, the maximum of soil biomass C,
接受日期：2010-04-08
收稿日期：2010-02-01
基金项目：国家烟草专卖局"重庆市山地特色烟叶生产技术体系研究”项目（200801001/200801002）资助。
作者简介：刘国顺（1954—），男，河南叶县人，教授，博士生导师，主要从事烟草裁培生理生化研究。E-mail：liugh1851@163.com 万方数据