植物营养与肥料学报2011,17(2)：317-324 Plant Nutrition and Fertilizer Science
田间条件下控制玉米开花前后根系性状的OTL定位蔡红光,2，刘建超"，米国华"，袁力行"，陈晓辉"，陈范骏，张福锁
（1中国农业大学资源与环境学院，农业部植物营养学重点实验室，教育部植物-土壤相互作用重点实验室，北京100193；
2吉林省农业科学院农业环境与资源研究中心，吉林长春130033）
摘要：在田间原位条件下，利用根系形态差异显著的自交系披478和武312为亲本构建的BC,F，群体，采用改进的 PLABQTL软件中的复合区间作图法对抽雄期（开花前10d)和滤浆初期（开花后15d)玉米根系性状的变化和地上部生物量进行QTL定位。并分析其遗传机制。结果表明，花前花后对根干重、总根长、侧根长、轴根长、轴根数等根系性状共检测出27个QTL，单个QTL贡献率为5.2%15.7%，其中在染色体替6.02和10.04区域同时检测到控制着地上部生物量、总根长、侧根长和轴根数等性状的QTLs，两个不同生育时期检测到的共同QTL共有8个。玉米花前花后控制根系生长的QTL因生长发育阶段不同而存在着特异性，而且对地上部生物量形成有重要贡献，这
为了解田间条件下根系的生长发育和进一步进行遗传改良莫定了遗传基础。关键词：玉米；抽雄期；灌浆初期；根系；QTL
中图分类号：S513.01
文献标识码：A
文章编号：1008-505X(2011)02-0317-08
QTLmappingforroottraitsaroundfloweringstageof
maize underfieldcondition
CAI Hong-guang'2, LIU Jian-chao', MI Guo-hua', YUAN Li-xing', CHEN Xiao-hui',
CHEN Fan-jun'', ZHANG Fu-suo"
(1 College of Resources and Environmental Sciences, China Agricultural Uninersity/Key Laboratory of Plant Nutrition Ministry of Agriculture/Key Laboratory of Plant-Soil Interactions, Minstry of Education, Bejing 100193, China
2 Research Center of Agricultural Emeironment and Resources,Jilin Academy of Agricultural Sciences
Changchun 130033, China)
Abstract : The purpose of this experiment was to study the variation of maize root around flowering for fully under standing the genetic mechanism of root development under field conditions and to offer theory basis for genetic improvement of root in high yield and quality agricultural system. A BC,F, population (187 lines) derived from Ye478 (with big root system) as the donor, and Wu312 (with small root system) as the recipient was developed. eaooaaeeaoeao PLABMQTL software at the tasseling stage (VT stage, 10 days before silking) and early filling stage (R2 stage, 15 days after silking) under field conditions. Totally, 27 QTLs are detected for controlling root dry weight (RDW), total root length (TRL), lateral root length (LRL), axial root length (ARL) and axial root number (ARN). The contribution of each QTL varies between 5.2%15.7%. Two important regions are detected on chromosome bin 6. 02 and 10. 04 with controlled shoot dry weight, total root length, lateral root length and axial root number, and eight common QTLs are found at the both growth stages. There are specific genetic mechanisms for different root types at pre and post flowering stages, and the QTLs detected for root related traits are important to the formation of shoot growth of maize, These QTLs are helpful to understand the genetic basis of maize root under the field condi-
收稿日期：2010-04-19
接受日期：2010-08-13
基金项目：国家自热科学基金（30600381）；国家基金创新群体项目（No.30821003）；公益性行业科研专项（200803030）资助。作者简介：蔡红光（1981—），男，吉林舒兰人，净士，研究方向为植物营养高效生理与遗传。E-mail：caibongrangl981@163.com
*通讯作者Tel;010-62734454，E-mail;caucfj@ cau.edu,cn
感谢Hobenheim大学JochenCReif提供改进的PLABQTL软件用于QTL分析(PALBMQTL)。万方数据