植物营养与肥料学报2013,19(2)：510-516 Plant Nutrition and Fertilizer Science
doi: 10. 11674/zwyf. 2013. 0229
于旱胁迫下丛枝菌根真菌对玉米生理生化
特性的影响秦子娴，朱敏，郭涛*（西南大学资源环境学院，重庆400716）
摘要：在土壤有效磷含量较高的条件下，研究了接种丛枝菌根真菌（arbuscularmycorrhizalfungus，AMF）对不同水分处理梯度（田间最大持水量的70%、45%和20%）条件下玉米生长和抗旱性的影响。结果表明，在三种不同水分处理条件下，接种AMF显著提高了玉米植株地上部的生物量，且对玉米地上部、地下部全磷含量有显著提高作用。水分胁迫显著降低了玉米叶片水势，增加了玉米叶片脯氨酸、丙二醛、过氧化物酶和过氧化氢酶含量，而不同水分梯度条件下接种AMF均显著提高了植株叶片水势，降低了脯氨酸含量，提高了叶片保护酶（过氧化物酶和过氧化氢酶)活性从而减少丙二醛的积累，减轻植物叶片膜脂过氧化的伤害。由此说明，接种AMF在土壤有效磷含量较
高的条件下能够与玉米根系形成良好的共生关系，提高玉米的抗旱性。关键调：丛枝菌根真菌；干早胁迫；生理生化；有效碳
中图分类号：S144.5；S513.01
文献标识码：A
文章编号：1008505X(2013)020510-07
Influence of mycorrhizal inoculation on physiological and biochemical
characteristicsofmaize（Zeamays）underwaterstress
QIN Zi-xian, ZHU Min, GUO Tao
(College of Resources and Emironment, Southaest Unitersity, Chongqing 400715, China)
Abstract: To study the arbuscular mycorrhiza inoculation on growth and drought resistance of maize (Zea mays) in soils with high phosphorus availability, a pot experiments was carried out by the inoculation of Glomus intraradices and Glomus etunicatum. There were three irrigation levels, 70% , 45% and 20% of soil field capacity. The results show that compare to the non-mycorrhizal treatment, the mycorrhizal inoculation increases the host plant shoot and the phosphorus contents of shoots and roots significantly. Hydraulic potential, the contents of prolin, POD and CAT of maize leaves are decreased under the treatments with 45% and 20% of soil field capacity. The mycorrhizal inoculation increases the hydraulic potential, POD and CAT activities and decreases prolin concentration of host plants which relieve membrane lipid peroxidation caused by water stress, It can conclude that arbuscular mycorrhiza fungus could form symbiosis with host plants and improve drought resisting ability even under high soil phosphorus availability
Key words: arbuscular mycorrhiza; water stress; physiology and biochemistry; available phosphorus
玉米是我国北方和西南山区的主要粮食作物之一，目前限制玉米生长及其产量的重要因素之一是干旱")。在我国干旱和半干旱地区约占国土面积的50%，主要分布于西部地区。据不完全统计，由于干旱造成的作物减产和植物生长减少远远超过了
其他逆境造成的总和(")。因此水分胁迫一直是农业研究的重点之一。
丛枝菌根（arbuscularmycorrhiza fungus，AMF）是分布于各种陆地生态系统中最广的一类内生菌根，它能够与大多数植物共生，目前已知世界上大约
收稿日期：201206-06
接受日期：2012-10-17
基金项目：中央高校基本科研业务费专项资金（XDJK2010B012)；国家自然科学基金项目（40701085）资助。
作者简介：秦子（1989—），女，四川达州人，硕士研究生，主要从事荫根生理生态方面的研究。E-mail；14345311@qq.com
*通信作者E-mail；guotaosd@swu.edu.cn
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