植物营养与肥料学报2014,20(1)：195-204 Joumal of Plant Nutrition and Fertilizer
doi:10.11674/zwyf.2014.0122
番茄 SIMIP基因参与转基因拟南芥的渗透调节
徐娜，辛士超，强晓晶，于国红，马雪峰，程宪国
（农业部植物营养与肥料重点实验室，中国农业科学院农业资源与农业区划研究所，北京100081）
摘要：本实验以野生型(WT)和转番茄SIMIP基因的拟南芥为材料，研究NaCI胁迫条件下对二者体内渗透调节特征的影响。结果发现，在NaCI胁迫下，转SIMIP基因的拟南芥细胞膜损伤程度较轻，组织渗透势显著降低，并保持较高的组织含水量，生长受抑制程度明显低于野生型植株。转基因植株体内Na*含量和Na'/K'比值显著低于野生型拟南芥，K*含量虽有所下降但仍显著高于野生型植株，面且在盐胁迫下脯氨酸和可溶性糖含量也高于野生型拟南芥，表明转人番茄SIMIP基因后的拟南芥，通过增加水分子的吸收，减少钠离子的进人，增加了细胞内脯氨酸和可溶性糖的含量，进而影响植物的有机渗透调节能力，与此同时可能通过离子化区隔机制以及与质膜Na/H* 逆向转运蛋白的相互作用更有效地调节细胞内外的无机离子交换能力，使植物更有效地抵御盐害，表明番茄SIMIP
水通道蛋白基因在植物盐胁追下具有重要的渗透调节作用。关键词：拟南芥；盐胁迫；水通道蛋白；渗透调节
中图分类号：S184
文献标识码：A
文章编号：1008505X(2014)010195-10
TomatoSlMiPgeneisinvolvedinosmoticadjustmentinthetransgenic
Arabidopsisthaliana
XU Na, XIN Shi-chao, QIANG Xiao-jing, YU Guo-hong, MA Xue-feng, CHENG Xian-guo
(Key Laboratory of Plant Nutrition and Fertilizer Science, Ministry of Agriculture/Institute of Agricultural Resources
and Regional Planning, CA4S, Bejing 100081, China)
Abstract: In this study, wild type( WT) and transgenic Arabidopsis thaliana carrying SIMIP gene were used to evaluate the change characteristics of osmotic adjustment under different levels of NaCl stress. The results show thal the transgenic Arabidopsis carrying SiMiP gene significantly decreases the damage of cell membrane and tissue osmotic potential compared to the wild type, and the transgenic Arabidopsis could maintain higher water content and better growth performance under the salt stress. The content of Na* and ratio of Na*/K* in the transgenic Arabidopsis are obviously lower than those in the wild type, while the content of K * in the transgenic Arabidopsis is higher than that in the wild type plants. In addition, the transgenic Arabidopsis has more accumulation of proline and soluble sugar compared to the wild type Arabidopsis, indicating that the transgenic Arabidopsis thaliana could enhance the organic solute adjustment ability by increasing the uptake of water molecular and decreasing the entrance of Na* which lead to increase in the content of proline and soluble sugar. Data seems to imply that the transgenic Arabidopsis can more effectively regulate the inorganic exchange by ion compartmentation and interaction with Na*/H* antiporter, which is located in plasma membrane. These suggest that the transgenic Arabidopsis shows stronger salt tolerance compared to the wild type, and Solanum lycopersicum MIP aquaporin plays an important role in plant osmotic adjustment under the slat stress.
Key words: Arabidopsis thaliana; salt stress; aquaporin; osmotic adjustment
收稿日期：2013-04-12
接收日期：2013-07-16
基金项目：国家转基因重大专项（2013ZX08002-005）资助
作者简介：徐娜（1988一），女，河南南阳人，硕士研究生，主要从事植物生理生化及养分高效利用分子机理方面的研究。
E-mail; xunasxnd@163. com。
万方数据
通信作者E-mail;chengxianguo@ tsinghua.org.cn