植物费养与肥料学报2011,17(1)：55-61 PlantNutritionandFertilizerScience
钾素对玉米茎腐病抗性反应中糖类物质代谢
的影响
李文娟"2，何萍2*，金继运？
（1中国农业科学院农业环境与可持续发展研究所，北京100081：
2中国农业科学院农业资源与农业区划研究所，农业部作物背养与施肥重点开放实验室，北京100081)
摘要：通过砂培试验比较人工接种禾谷镰刀菌（Fusariumgraminearum）后，不同钾处理对玉米幼根中糖含量及糖代谢关键酶活性和基内表达的影响。结果表明，不施钾处理中，接菌比未接菌处理天米幼根中蔗糖和能翻糖含量显着降低，且蔗糖的下降速率高于德靠糖的下降速率。随并病原菌人侵时的延长，不施钾处理能益糖与盖比值显着升高，接渊8d后，缺钾幼根需葡糖与熊糖的比值是充足供钾幼根的近10倍。此外，钾素有利于病原菌入侵，幼根中蔗精合成酶(SS)和能精磷酸合成酶(SPS)活性提高，s和sps基因表达量增加；耐缺钾处理中，ss基因的表达量儿乎没有发生变化，sps基因的表达量在接后6d才有所增加。说明在病原菌人侵后，钾素可以通过调节受
侵染组织糖代谢相关酶的活性，协调受仪染部位糖代谢过程，增强抗病能力。关键调：钾；玉米茎腐病；抗性；精代谢；酵活性
中图分类号：S513.01
文献标识码：A
文章编号：1008505X(2011)010055-07
Effectof potassium onsugarmetabolisminresistantresponsetocorn stalk rot
LI Wen-juan'-?, HE Ping'', JIN Ji-yun*
(I Institute of Ensironment and Sustainable Derelopment in Agriculture, Chinese Academy of Agricultural Sciences,
Bejing 100081, China;
2 Institute of Agricultural Resowrces and Regional Planning, Chinese Academy of Agricultural Sciences/ Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization, Bejing 100081, China)
Abstract: The effects of potassium on sucrose and glucose content, sugar metabolism related enzyme activity and the expression of related genes in maize root infected with Fusarium graminearum were studied under sand-culture experiment. The results showed that under K stress, pathogen infection led to significant decrease of sucrose and glucose contents. The sucrose content declined more quickly than that of glucose. With the process of infection, the ratio of glucose to sucrose increased in no potassium applied treatment; 8 days after infection, the ratio was 10 times higher in K deficient roots than in K adequate roots. Besides, after inoculation, the activity of sucrose phos-phate synthase (SPS) and sucrose synthase (SS) were increased as well as the gene expression of sps and ss in po tassium treated maize root. In K-treatment, sps gene expresion increased in 6 days after inoculation, and no change of ss expression was observed in K-stressed root. This study demonstrated that K could enhance maize resist-ance to stalk rot by regulating sugar metabolism related enzyme, consequently, maintain the optimal sugar metabo-lism in infected position, and enhance the resistance to stalk rot.
Key words: potassium: corm stalk rot; resistance; sugar metabolism; enzyme activity
收稿日期：2010-06-17
接受日期：2010-07-29
（6）（20909（1800）
(2007CB109306）；北京市科技新计划项H(2005A60）；国际植物营养研究所（IPNI)项目资助。
作奢简介：李文（1982一），女，山两太原人，博士，主要从事植物背养生理研究。E-mail；liwenjuan972@sohu.con
通讯作者Tel;010-82108000,E-mail：phecas,ac.cn
本文为2010中国植物营养与肥料学会学术年会会议论文。万方数据