南方农业学报Journal of Southem Agriculture2016,47(6)：889894 ISSN 20951191; CODEN NNXAAB
D0I:10.3969/j:issn.20951191.2016.06.889
3种东方百合离体培养
闫海霞*，蒋月喜*，邓杰玲，黄昌艳，王晓国，何荆洲，下朝阳*
(广西农业科学院花卉研究所，南宁530007)
http ://www.nfnyxb.com
摘要：目的研究3种东方百合离体培养技术，筛选出适宜其离体培养的统一培养基，为东方百合工厂化育苗提供技术保障。【方法]以3种东方百合提伯、索邦和西伯利亚的鳞片为外植体，筛选适宜的灭菌方法；在MS基本培养基上添加不同种类和不同浓度的激素，筛选适宜的增殖培养基和生根培养基，进一步筛选最适移裁基质。【结果适宜3 种东方百合外植体灭菌的较好方法是：75%酒精灭菌15s结合升汞灭菌12min；愈伤诱导和分化的适宜培养基为MS； 0%9%%9000 移裁的最佳基质为泥炭：珍珠岩（体积比)=2:1，提伯、索邦和西伯利亚的移裁成活率分别为97.34%、98.00%和 100.00%。【结论]建立的3种东方百合离体快繁体系适用于东方百合种球工厂化生产
关键词：东方百合；外植体；离体培养；培养基
中图分类号：S682.2
文献标志码：A
文章编号：2095-1191(2016)060889-06
Invitroculturefor3varietiesoforientallily
YAN Hai-xia', JIANG Yue-xi', DENG Jieling, HUANG Chang-yan, WANG Xiao-guo,
HE Jing-zhou,BU Zhaoyang*
(Flower Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China)
Abstract: [Objective JThe in vitro tissue culture technique of oriental lily was studied, and the unified culture medi-um was screened, in order to provide theoretical and technical support for industrialized seedling production. [MethodJThe bulb scales of three lily cultivars viz., Tiber, Sorbonne and Siberi were used as explants, which were cultured in minima medium(MS) supplemented with different hormones. [Result]The results showed that, the best sterilization method was as follow: 0.1% HgCl for 12 min, followed by 75% alcohol for 15 s. The suitable culture medium for callus induction and differentiation was MS. The optimum medium for subeulture multiplication was MS+0.5 mg/L6BA +0.5 mg/L NAA, and the multiplication coefficients of Tiber, Sorbonne and Siberi were 3.32, 3.32 and 3.00, respectively. The optimum culture medium for rooting culture was MS+0.1 mg/L IBA, and the rooting rates of Tiber, Sorbonne and Siberi were 96.33% , 92.67% , 93.67% respectively. The optimum substrate for transplanting was peat mixed with perlite in the volume propor-tion of 2:1, the survival rates of Tiber, Sorbonne and Siberi were 97.34%, 98.00% and 100.00%, respectively. [Conclu-sion JThe tissue culture and rapid propagation system for 3 varieties of oriental lily is established, and which is applicable to factory production of lily bulb.
Key words: oriental lily; explant; in vitro culture; medium
0引言
【研究意义】百合（Liliumspp.）为百合科（Lili-aceae)百合属（LiliumL.）多年生植物。近年来，切花百合在市场上极受欢迎，应用前景广阔，尤其是东方百
收稿日期：2016-01-21
合在国内外市场的需求量极大。在百合切花生产上，我国商品化种球主要依靠国外进口，存在成本高及携带他国病毒风险高等问题。因此，优化百合种球繁育技术是降低成本、避免他国病毒入侵的有效途径。分
（0（890086
广西农业科学院创新团队项目（2015YT89）
作者简介：**为通讯作者，下朝阳（1966-），研究员，主要从事观赏植物生物技术研究工作，E-mail:yangnv@126.com。*为并列第一
作者，对论文的贡献相等：国海霞（1981-），主要从事花卉新品种选育与示范推广工作，E-mail：819307232@qq.com；蒋月喜（1963-），主要从事花卉新品种选育及示范推广工作，Email：13807818029@163.com