第35卷，第5期 2015年5月
光谱学与光谱分析 Spectroscopy and Spectral Analysis
Vol.35,No.5-ppl222-1227
May,2015
RapidIsolationofPhenolDegradingBacteriabyFourier
Transform Infrared (FTIR) Spectroscopy
LI Fei',SONG Wen-jun'*,WEI Ji-ping",WANG Su-ying',LIU Chong-ji
1. Tianjin University of Commerce , Tianjin 300134 , China 2. Tianjin University , Tianjin 300027, China
3. Arizona State University , Tempe , Arizona 85287, USA
Abstract Phenol is an important chemical engineering material and ubiquitous in industry wastewater, its ex-istence has become a thorny issue in many developed and developing country . More and more stringent stand-ards for effluent all over the world with human realizing the toxicity of phenol have been announced . Many ad-vanced biological methods are applied to industrial wastewater treatment with low cost , high efficiency and no secondary pollution, but the screening of function microorganisms is certain cumbersome process . In our study a rapid procedure devised for screening bacteria on solid medium can degrade phenol coupled with attenuated total reflection fourier transform infrared (ATR-FTIR) which is a detection method has the characteristics of efficient , fast , high fingerprint were used . Principal component analysis (PCA ) is a method in common use to extract fingerprint peaks effectively , it couples with partial least squares (PLS ) statistical method could estab-lish a credible model . The model we created using PCA-PLS can reach 99, 5% of coefficient determination and validation data get 99. 4% , which shows the promising fitness and forecasting of the model . The high fitting model is used for predicting the concentration of phenol at solid medium where the bacteria were grow n . The highly consistent result of two screening methods , solid cultural with ATR-FTIR detected and traditional liq uid cultural detected by GC methods , suggests the former can rapid isolate the bacteria which can degrade sub-strates as well as traditional cumbersome liquid cultural method . Many hazardous substrates widely existed in industry w astewater , most of them has specialize fingerprint peaks detected by ATR-FTIR , thereby this detec-ted method could be used as a rapid detection for isolation of functional microorganisms those can degrade many other toxic substrates .
Keywords Industrial sewage :; Phenol biodegradation : Screening : ATR-FTIR ; PLS model
中图分类号：0657.3
Introduction
文献标识码：A
DOI;10.3964/j.issn.1000-0593(2015)05-1222-06
are applied to degrade organics . Physical methods such as ex-traction , adsorption and ion exchange are high cost . Chemical methods like oxidation (Spiker et al. 1992 ) and precipitation
Industrial sewage treatment is tremendous tricky because complex components within sewage, such as phenols , amines and long-chain alkanes, are biotoxic and hard to degrade in nature. Normally + physical chemical and biological methods
Received : 2014-10-14 ; accepted : 2015-01-22
(Cowling, S. J. et al. 1992), are also high cost and may cause secondary pollution. Comparing with physical and chemical methods , biological methods through bacteria de-grading seemspromising to decrease cost and increase efficien-
Foundation item: Tianjin Science and Technology Support Program (13ZCZDSF00600 ), National Natural Scienee Foundation of China
(31270050)
Biography : LI Fei, (1989—), a graduate student at Tianjin University of Commerce
Corresponding author
e-mail : songwenjur tjcu .edu -cn
e-mail : sam-life@ 163.com