植物背养与肥料学报2010,16(2)：395-399 Plant Nutrition and Fertilizer Science
大豆鼓粒期对肥料氮的吸收与分配研究
金喜军"，马春梅"，龚振平2*，姚玉波"，邸伟
（1东北农业大学农学院，黑龙江哈尔滨150030；2国家大豆工程技术研究中心，黑龙江省哈尔滨150030）
摘要：采用"N分阶段标记的方法，研究了鼓粒期间大互植株对氮素的同化吸收，尤其是肥料氮在各器官中的积累与分配。结果表明：1)5次标记试验期间积累的鼠素总量为291.4m/plant，占标记试验V结束时（鼓税术期)大豆植株氮素积累总量的48.9%，说明蚊粒期间的氮索积累对于大豆氮素积累具有重要作用；2）从鼓粒始期到鼓粒末期，大豆植株氮系阶段积累量急剧降低，由标记试验I的109.5mg/plant下降到标记试验V的19.6mg/plant；3)鼓粒期大豆植株所吸收的氮素主要来自根瘤固氮，该部分氮占总同化量的67.4%，葡肥料氮只占32.6%；4)根瘤固氮和肥料氮的同化量均随着数粒进程的推进而下降，分别由标记试验I的67.5和42.0mg/plant下降到标记试验V的10.8和 8.8m/plant；5)5次标记试验中，不同器官中肥料鼠的积累量均表现出一致的规律性，为子粒>叶片>基>根系>英
皮，并且分配到子粒中的肥料氮比例是逐新增加的，由标记试验1的27.9%增加到标记试验V的85.0%。关键词：大豆；鼓粒期；肥料氮；粮瘤固氮
中图分类号：S145.7
文献标识码：A
文章编号：1008505X(2010)02-0395-05
Study on fertilizer-N absorption and distribution of soybean [Glycine max (L).]
during theseed-fillingperiod
JIN Xi-jun', MA Chun-mei', GONC Zhen-ping', YAO Yu-be', DI Wei'
(I Agronomy College, Northeast Agricultwre Unitersity, Harbin, Heilongjiang 150030, China;
2 National Researoh Soybean Engineering and Technology Center, Harbin, Heilongjiang 150030, China)
Abstract: This experiment was conducted by using '5N labeling method with staged design to study nitrogen assimilation and absorption by the soybean plant during the seed-flling period, especially the distribution of fertilizer N among differ-ent organs of soybean. The results show that total nitrogen accumulation during the five labeling experiments (025 d af-ter beginning of seed-filling) plays an important role in nitrogen accumulation in soybean plant, which is about 291.4 mg/plant, occupies 48.9% to the total nitrogen accumulation in the labeling experiment V. The nitrogen accumulation is decreased sharply during the seed filling period, which is from 109.5 mg/plant in the labeling experiment I (0-5 d after beginning of seed-filling) to 19.6 mg/plant in the labeling experiment V . Nodulation-N is the main source of the plan assimilated N, which is about 67.4% , and nodulations-N accumulation is decreased from 67.5 mg/plant in the la-beling experiment I to 10.8 mg/plant in the labeling experiment V . Fertilizer-N accumulation is also decreased with the seed-flling proces8, from 42.0 mg/plant in the labeling experiment I to 8.8 mg/plant in the labeling experiment V . Ferilizer-N accumulation in different organs of soybean in different labeling experiments exhibits the same orders: seeds > leaves > stems > roots > pod walls, and the proportion of seed N accumulation from the fertilizer is increased from 27.9% to 85.0% during the labeling period.
Key words: soybean; seed filling stage; fertilizer-N; nodulation-N
收稿日期：2009-03-23
接受日期：2009-07-14
基金项目：黑龙江省教育厅骨干教师项目：国家"十一五"科技支挥计划（2006BAD21B01，2007BAD89B05）资助。
作者简介：金喜军（1979—），男，黑龙江人，博士研究生，主要从事作物生理与生物技术研究。E-msil：shaoxiang1979@yahoo.cn
通讯作者E-mail:gpyx2004@163.com
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