植物营养与肥料学报2016,22(1）：236-244 Journal of Plant Nutrition and Fertilizer
doi:10.11674/zwyf.14413 http://www.plantnutrifert.org
MicroRNA调节豆科作物营养胁迫响应的研究进展
徐锋，王金祥*
（亚热带农业生物资源保护与利用国家重点实验室，华南农业大学农学院，广州510642）
摘要：micorRNA（miRNA）是一类长度为20～24个核苷酸的非编码小RNA（smallRNA，sRNA）在植物生长发育、生物和非生物胁迫响应方面起十分重要作用。越来越多的证据表明，miRNA在植物适应养分胁迫方面起重要的调节作用。豆科植物是一类具有生物固氮能力的植物，为人类提供蛋白和食用油，显然土壤养分胁迫会抑制豆科作物生长发育而降低产量。过去数十年对于miRNA介导模式植物拟南芥和水稽养分胁迫响应的研究较多，但近年来有关豆科作物养分胁迫相关的miRNA报道在增加。近年研究结果表明，miRNA通过对靶基因的调节在豆科植物适应营养胁迫中起关键作用，如感受外界养分状态的改变及维持体内养分的动态平衡。本文综述了近年来 miRNA介导豆科作物适应养分胁迫的研究进展，主要对磷、氮、硫、铁、铜、钙等养分亏缺或毒害反应的调控，讨
论了miRNA调节豆科作物适应养分胁迫的机理，并对今后豆科作物miRNA的研究做出了展望。关键词：miRNA；营养胁迫；豆科作物
中图分类号：Q945.12；S565.1
文献标识码：A
文章编号：1008-505X(2016)01023609
AdvancesonthestudyofmicroRNA-mediatedresponsesto
nutrientstressinlegumecrops
XU Feng, WANG Jin-xiang
(State Key Laboratory for Conseruation and Utilization of Subtropical Agro-bioresources/ College of Agriculture, South China Agricultural University, Guangzhou 510642, China)
Abstract: MicroRNA (miRNA) is one kind of non-coding small RNA (sRNA), and has 20 -24 nucleotides in length, and plays important roles in plant growth development and in response to biotic and abiotic stress. Many evidences have shown that miRNA is crucial modulator in adaptations of plant to nutrient stress. Legume plants could fix nitrogen from atmosphere and provide proteins as well as edible oil for human being. Nutrient stresses in soils obviously inhibit legume growth and development, and result in a decrease in yield. In past decades, most studies on mediation roles of miRNA in responses to nutrient stresses in Arabidopsis and rice have been characterized, respectively. However, recent studies have witnessed emerging reports on the functions of miRNA in response to nutrient stress in legumes, and revealed crucial roles of miRNAs in adaptations of legumes to various adverse nutrient conditions via modulating activity of target genes such as sensing alteration of nutrient status and fine-tuning nutrient homeostasis. In this review, the regulation role of miRNAs in response to different nutrient stresses was reviewed, especially in response to the stresses from phosphorus (P), nitrogen (N), sulphur (S), iron (Fe), copper (Cu) deficiencies and calcium (Ca) toxicity, and the mechanisms of miRNAs involved in the adaptations of legume to different kinds of nutrient stress were discussed, and the perspective of research on
nutrient-related miRNA in legume was outlined in the near future. Key word: miRNA; nutrient stress; legume crops
收稿日期：2014-09-02
接受日期：2014-10-10
网络出版日期：2015-07-02
基金项目：农业部转基因专项(2014ZX0800928B）；科技部重大基础研究专项基金973项目（2011CB100301）资助。
作者简介：徐锋（1986一），男，广东云浮人，博士研究生，主要从事植物营养分子生物学方面的研究。E-mail：june92634@126.com
*通信作者E-mail：jinxwang@scau.edu.cn