第18卷
第5期
2010年5月文章编号
1004-924X(2010)05-1028-07
光学精密工程
Optics and Precision Engineering
线扫描准共焦荧光成像杨彬，刘乐，刘智毅，马绥华，种心原，何永红
（清华大学深圳研究生院光学检测与成像实验室，广东深圳·518055）
Vol.18No.5
May2010
摘要：为了克服目前生物芯片荧光检测方法中诸如系统结构复杂、检测速度慢、灵数度低、成本高等缺点，提出了种新
型生物芯片荧光检测方法一
线扫描准共焦荧光成像法，并搭建了初步原理性装置。用线扫描代替共聚焦中的点扫描，
将二维扫描变为一维扫描，在保持高灵敏度的同时，增加了探测速度，简化了系统，降低了成本。为了验证方法的可行性，使用搭建的原理性装置对手工点样的低密度DNA生物芯片进行了荧光成像检测。实验结果显示，系统的空间分辨率<18μm，在使用像素平均法降噪后，测量浓度为0.03μmol/1的探针溶液所得信噪比为5.5×10°。这项技术综合了面成像检测方法的低成本、结构简单的优势和点共焦方法其有的高分辨率的优点，适合在实验室中对生物芯片进行检测研究。
关键
词：线扫描；生物芯片，微降列；荧光成像
中图分类号：TN247
文献标识码：A
doi;10. 3788/OPE. 20101805.1028
Line-scanning quasi-confocal fluorescence imaging
YANG Bin, LIU Le, LIU Zhi-yi, MA Sui-hua, CHONG Xin-yuan, HE Yong-hong(Laboratory of Optical Imaging and Sensing,GraduateSchool atShenzhen,
TsinghuaUniversity,Shenzhen518055,China)
Abstract: In order to overcome the drawbacks of traditional fluorescence imaging methods and to a chieve a biochip fluorescence imaging technique with high sensitivity and low .costs,a novel biochip flu orescence imaging technique,line-scanning quasi-confocal fluorescence imaging method,was proposed and a prototype system was set up. By using a line scanning to replace a point scanning and changing the 2-D scan into the 1-D scan, the system can provide higher sensing speeds, simpler structures and lower costs while maintaining the high sensitivity. To verify the feasibility of the method, the proto-type system was used to sense the fluorescence image of a manually prepared low density DNA mi-croarray. Experimental results indicate that the spatial resolution of the system is better than 18 μm, and the Signal to Noise Ratio(SNR) of the system is 5. 5 X 1o’ by using the pixel-averaging method to reduce noises, By combining the low cost and simple structure of the area imaging method with the high sensitivity of the confocal point-scanning imaging method,the technique is suitable for the biochip study in laboratories.
Key words: line scanning; biochip; microarray; fluorescence imaging
收稿日期：2009-05-31；修订日期：2009-07-27.
基金项目：国家863高技术研究发展计划资助项日（No.2006AA06Z402）；国家自然科学基金资助项目（No.
30770592,60608019)