DB61/T 1134-2018
番茄黄化曲叶病毒检验鉴定方法
Detection and identification of Tomato yellow leaf curl virus
2018-05-10实施
2018-04-10发布
前 言............................................................................................................................................................1
1 范围.............................................................................................................................................................. 1
2 规范性引用文件.......................................................................................................................................... 1
3 番茄黄化曲叶病毒基本信息...................................................................................................................... 1
4 方法原理...................................................................................................................................................... 1
5 实验用仪器设备、用具及试剂.................................................................................................................. 1
6 取样和样品制备.......................................................................................................................................... 2
7 检测方法...................................................................................................................................................... 2
8 结果判定...................................................................................................................................................... 2
9 结果记录与样品、资料保存...................................................................................................................... 2
10 安全............................................................................................................................................................ 3
附录 A(规范性附录) TYLCV 相关资料 .................................................. 4
附录 B(规范性附录) 双抗体夹心酶联免疫吸附测定方法.................................. 7
附录 C(规范性附录) 普通 PCR 检测方法............................................... 11
附录 D(规范性附录) 实时荧光 PCR 检测方法........................................... 14
附录 E(规范性附录) 生物学接种..................................................... 16
本标准按照GB/T 1.1—2009给出的规则起草。
本标准由陕西省农业厅提出并归口。