GB/T 38551—2020
植物品种鉴定MNP标记法
Identification of plant varieties—MNP marker method
2020-03-06 发布
2020-10-01 实施
目 次
前言...........................................1
1范關..........................................1
2规范性引用文件.....................................1
3术语和定义.......................................1
4原理..........................................2
5试剂或材料.......................................2
6仪器设备........................................2
7测定步骤........................................2
7.1操作要求.....................................2
7.2取样.......................................2
7.3 DNA 提取..................................2
7.4多重PCR扩増与文库构建.............................2
7.5 高通fl：测序.................................2
7.6测序数据质最控制.................................3
8结果分析........................................3
8.1结果计算.....................................3
8.2结果判定.....................................3
附录A （规范性附录）MNP标记引物...........................5
A.1水稻MNP标记引物................................5
A.2玉米MNP标记引物...............................35
A.3大豆MNP标记引物...............................63
A.4棉花MNP标记引物...............................78
A.5 花生MNP标记引物 ........................... 109
A.6谷子MNP标记引物..............................124
A.7西瓜MNP标记引物..............................148
A.8甜瓜MNP标记引物..............................171
A.9黄瓜MNP标记引物..............................192
A.10艾草MNP标记引物.............................208
A.11番茄MNP标记引物.............................218
A.12 辣椒MNP标记引物............................235
A.13白菜MNP标记引物.............................260
A.14 龙眼MNP标记引物............................275
A.15荔枝MNP标记引物.............................294
A.16 勝猴桃MNP标记引物...........................312
前 言
本标准按照GB/T 1.1—2009给岀的规则起草.
本标准由中国标准化研究院提岀并归口